![]() Acetylation of Protein N-term, oxidation of methionine, and phosphorylation of serine, threonine and tyrosine were set as variable modifications, and they were also included in protein quantification. Multiplicity was set to 2, with Arg10 and Lys6 as heavy labels. Data were searched against RefSeq mouse database (58513 sequences, downloaded Oct. ![]() Raw files from each passage (biological replicate) were searched together to generate a combined result. Identification and quantification were done by MaxQuant (version 1.5.2.8). Cells were harvested, equally pooled and subjected to offline high-pH fractionation based two dimensional LC-MS/MS analysis (Q-Exactive). Cells were stimulated for four days with angiotensin-2 (ANGII, 1nM) to both apical and basolateral sides. Four passages of labelled cells were used to generate four biological replicates for statistical analysis. MpkDCT cells were cultured in heavy SILAC medium (Lys+6, Arg+10) while mpkCCD cells were cultured in light SILAC medium (Lys+0, Arg+0). ![]() See others: Control condition Long-term dDAVP treatment This database contains differential proteome quantified from Stable Isotope Labeling with Amino acids in Cell culture (SILAC) based quantitative proteomic study between cultured mouse kidney distal convoluted tubule cells (mpkDCT) and cortical collecting duct cells (mpkCCD) cells following four days treatment with 1 nM of angiotensin-2 to both apical and basolateral sides.
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